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Article Dans Une Revue FEMS Microbiology Letters Année : 2006

Isolationand characterization of functional insertion sequences of rhizobia

Résumé

Rhizobia are a group of bacteria that form nodules on the roots of legume host plants. The sequenced genomes of the rhizobia are characterized by the presence of many putative insertion sequences (IS) elements. However, it is unknown whether these IS elements are functional and it is therefore relevant to assess their transposition activity. In this work, several functional insertion sequences belonging to the IS1256, IS3, IS5, IS166, and IS21 families were captured from Rhizobium tropici, Rhizobium sp. NGR234 and Sinorhizobium meliloti, using pGBG1 as a trapping system. In silico analysis shows that homologs of rhizobia mobile elements are present in distantly related genomes, suggesting that Rhizobium IS elements are prone to genetic transfer.
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Dates et versions

hal-00113510 , version 1 (13-11-2006)

Identifiants

  • HAL Id : hal-00113510 , version 1
  • PRODINRA : 251978

Citer

Ismael Hernandez-Lucas, Jose Augusto Ramirez-Trujillo, Miguel Angel Gaitan, Xianwu Guo, Margarita Flores, et al.. Isolationand characterization of functional insertion sequences of rhizobia. FEMS Microbiology Letters, 2006, 261, pp.25-31. ⟨hal-00113510⟩
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