Impact of the N-terminal amino acid on targeted protein degradation. - CNRS - Centre national de la recherche scientifique Accéder directement au contenu
Article Dans Une Revue Biol Chem Année : 2006

Impact of the N-terminal amino acid on targeted protein degradation.

Résumé

The N-terminus of any protein may be used as a destabilization signal for targeted protein degradation. In the eukaryotic cytosol, the signal - the so-called N-degron--is recognized for degradation by (i) the N-end rule, a well-described degradation process involving epsilon-ubiquitination; or (ii) N-terminal ubiquitination, a more recently described pathway. Dedicated E3 ubiquitin ligases known as N-recognins then act on the protein. The proteolytic pathways involve ATP-dependent chambered proteases, such as the 26S proteasome in the cytosol, which generate short oligopeptides. The N-terminus of the polypeptide chain is also important for post-proteasome degradation by specific aminopeptidases, which complete peptide cleavage to generate free amino acids. Finally, in each compartment of the eukaryotic cell, N-terminal methionine excision creates a variety of N-termini for mature proteins. It has recently been shown that the N-terminal methionine excision pathway has a major impact early in targeted protein degradation.

Dates et versions

hal-00118264 , version 1 (04-12-2006)

Identifiants

Citer

Thierry Meinnel, Alexandre Serero, Carmela Giglione. Impact of the N-terminal amino acid on targeted protein degradation.. Biol Chem, 2006, 387 (7), pp.839-51. ⟨10.1515/BC.2006.107⟩. ⟨hal-00118264⟩
22 Consultations
0 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More