Gene expression profiling in human gastric mucosa infected with Helicobacter pylori. - CNRS - Centre national de la recherche scientifique Accéder directement au contenu
Article Dans Une Revue Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc Année : 2007

Gene expression profiling in human gastric mucosa infected with Helicobacter pylori.

Résumé

Pathogenic mechanisms associated with Helicobacter pylori infection enhance susceptibility of the gastric epithelium to carcinogenic conversion. We have characterized the gene expression profiles of gastric biopsies from 69 French Caucasian patients, of which 43 (62%) were infected with H. pylori. The bacterium was detected in 27 of the 42 antral biopsies examined and in 16 of the 27 fundic biopsies. Infected biopsies were selected for the presence of chronic active gastritis, in absence of metaplasia and dysplasia of the gastric mucosa. Infected antral and fundic biopsies exhibited distinct transcriptional responses. Altered responses were linked with: (1) the extent of polymorphonuclear leukocyte infiltration, (2) bacterial density, and (3) the presence of the virulence factors vacA, babA2, and cagA. Robust modulation of transcripts associated with Toll-like receptors, signal transduction, the immune response, apoptosis, and the cell cycle was consistent with expected responses to Gram-negative bacterial infection. Altered expression of interferon-regulated genes (IFITM1, IRF4, STAT6), indicative of major histocompatibility complex (MHC) II-mediated and Th1-specific responses, as well as altered expression of GATA6, have previously been described in precancerous states. Upregulation of genes abundantly expressed in cancer tissues (UBD, CXCL13, LY96, MAPK8, MMP7, RANKL, CCL18) or in stem cells (IFITM1 and WFDC2) may reveal a molecular switch towards a premalignant state in infected tissues. Tissue microarray analysis of a large number of biopsies, which were either positive or negative for the cag-A virulence factor, when compared to each other and to noninfected controls, confirmed observed gene alterations at the protein level, for eight key transcripts. This study provides 'proof-of-principle' data for identifying molecular mechanisms driving H. pylori-associated carcinogenesis before morphological evidence of changes along the neoplastic progression pathway.Modern Pathology advance online publication, 20 July 2007; doi:10.1038/modpathol.3800930.

Dates et versions

hal-00166154 , version 1 (01-08-2007)

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Véronique J Hofman, Chimène Moreilhon, Patrick D Brest, Sandra Lassalle, Kevin Le Brigand, et al.. Gene expression profiling in human gastric mucosa infected with Helicobacter pylori.. Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc, 2007, ⟨10.1038/modpathol.3800930⟩. ⟨hal-00166154⟩
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