Single-cell protein analysis of a single mouse embryo by two-dimensional capillary electrophoresis. - CNRS - Centre national de la recherche scientifique Accéder directement au contenu
Article Dans Une Revue Journal of Chromatography Année : 2006

Single-cell protein analysis of a single mouse embryo by two-dimensional capillary electrophoresis.

Melissa M Harwood
  • Fonction : Auteur
Md Abul Fazal
  • Fonction : Auteur
Norman J Dovichi
  • Fonction : Auteur

Résumé

The characterization of protein expression from a single-cell mouse embryo using two-dimensional capillary electrophoresis (2D-CE) is described. These zygotes were obtained from Hsf1 gene knockout mice. Single zygotes were lysed off-column and proteins were fluorescently labeled using the fluorogenic dye 3-(2-furoyl)quinoline-2-carboxaldehyde (FQ). After injection, analytes were separated first according to molecular weight using capillary sieving electrophoresis (CSE) and then by micellar electrokinetic capillary chromatography (MEKC) to obtain protein expression fingerprints. Analytes were detected in a sheath flow cuvette using laser-induced fluorescence. In a 1-h 2D-CE separation, over 100 components were resolved with a spot capacity of 380.

Dates et versions

hal-00170675 , version 1 (10-09-2007)

Identifiants

Citer

Melissa M Harwood, Elisabeth S Christians, Md Abul Fazal, Norman J Dovichi. Single-cell protein analysis of a single mouse embryo by two-dimensional capillary electrophoresis.. Journal of Chromatography, 2006, 1130 (2), pp.190-4. ⟨10.1016/j.chroma.2006.05.052⟩. ⟨hal-00170675⟩
17 Consultations
0 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More