Specific immunoassays for placental alkaline phosphatase as a tumor marker. - CNRS - Centre national de la recherche scientifique Accéder directement au contenu
Article Dans Une Revue Journal of Biomedicine and Biotechnology Année : 2006

Specific immunoassays for placental alkaline phosphatase as a tumor marker.

Résumé

Human placental (hPLAP) and germ cell (PLAP-like) alkaline phosphatases are polymorphic and heat-stable enzymes. This study was designed to develop specific immunoassays for quantifying hPLAP and PLAP-like enzyme activity (EA) in sera of cancer patients, pregnant women, or smokers. Polyclonal sheep anti-hPLAP antibodies were purified by affinity chromatography with whole hPLAP protein (ICA-PLAP assay) or a synthetic peptide (aa 57-71) of hPLAP (ICA-PEP assay); the working range was 0.1-11 U/L and cutoff value was 0.2 U/L EA for nonsmokers. The intra- and interassay coefficients of variation were 3.7%-6.5% (ICA-PLAP assay) and 9.0%-9.9% (ICA-PEP assay). An insignificant cross-reactivity was noted for high levels of unheated intestinal alkaline phosphatase in ICA-PEP assay. A positive correlation between the regression of tumor size and EA was noted in a child with embryonal carcinoma. It can be concluded that ICA-PEP assay is more specific than ICA-PLAP, which is still useful to detect other PLAP/PLAP-like phenotypes.

Dates et versions

hal-00172079 , version 1 (14-09-2007)

Identifiants

Citer

Sérvio T Stinghen, Juliana F Moura, Patrícia Zancanella, Giovanna A Rodrigues, Mara A Pianovski, et al.. Specific immunoassays for placental alkaline phosphatase as a tumor marker.. Journal of Biomedicine and Biotechnology, 2006, 2006 (5), pp.56087. ⟨10.1155/JBB/2006/56087⟩. ⟨hal-00172079⟩
44 Consultations
0 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More