Activator-mediated recruitment of the MLL2 methyltransferase complex to the beta-globin locus. - CNRS - Centre national de la recherche scientifique Accéder directement au contenu
Article Dans Une Revue Molecular Cell Année : 2007

Activator-mediated recruitment of the MLL2 methyltransferase complex to the beta-globin locus.

Celina Demers
  • Fonction : Auteur
Chandra-Prakash Chaturvedi
  • Fonction : Auteur
Jeffrey A Ranish
  • Fonction : Auteur
Gaetan Juban
  • Fonction : Auteur
Patrick Lai
  • Fonction : Auteur
Ruedi Aebersold
  • Fonction : Auteur
F Jeffrey Dilworth
  • Fonction : Auteur
Mark Groudine
  • Fonction : Auteur
Marjorie Brand
  • Fonction : Auteur

Résumé

MLL-containing complexes methylate histone H3 at lysine 4 (H3K4) and have been implicated in the regulation of transcription. However, it is unclear how MLL complexes are targeted to specific gene loci. Here, we show that the MLL2 complex associates with the hematopoietic activator NF-E2 in erythroid cells and is important for H3K4 trimethylation and maximal levels of transcription at the beta-globin locus. Furthermore, recruitment of the MLL2 complex to the beta-globin locus is dependent upon NF-E2 and coincides spatio-temporally with NF-E2 binding during erythroid differentiation. Thus, a DNA-bound activator is important initially for guiding MLL2 to a particular genomic location. Interestingly, while the MLL2-associated subunit ASH2L is restricted to the beta-globin locus control region 38 kb upstream of the beta(maj)-globin gene, the MLL2 protein spreads across the beta-globin locus, suggesting a previously undefined mechanism by which an activator influences transcription and H3K4 trimethylation at a distance.

Domaines

Génétique

Dates et versions

hal-00176493 , version 1 (03-10-2007)

Identifiants

Citer

Celina Demers, Chandra-Prakash Chaturvedi, Jeffrey A Ranish, Gaetan Juban, Patrick Lai, et al.. Activator-mediated recruitment of the MLL2 methyltransferase complex to the beta-globin locus.. Molecular Cell, 2007, 27 (4), pp.573-84. ⟨10.1016/j.molcel.2007.06.022⟩. ⟨hal-00176493⟩
34 Consultations
0 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More