Polyglutamylation is a posttranslational modification with a broad range of substrates. - CNRS - Centre national de la recherche scientifique Accéder directement au contenu
Article Dans Une Revue Journal of Biological Chemistry Année : 2007

Polyglutamylation is a posttranslational modification with a broad range of substrates.

Résumé

Polyglutamylation is a posttranslational modification that generates lateral acidic side chains on proteins by sequential addition of glutamate amino acids. This modification was first discovered on tubulins and it is important for several microtubule functions. Besides tubulins, only the nucleosome assembly proteins NAP1 and NAP2 have been shown to be polyglutamylated. Here, using a proteomic approach, we identify a large number of putative substrates for polyglutamylation in HeLa cells. By analyzing a selection of these putative substrates, we show that several of them can serve as in vitro substrates for two of the recently discovered polyglutamylases, TTLL4 and TTLL5. We further show that TTLL4 is the main polyglutamylase enzyme present in HeLa cells and that new substrates of polyglutamylation are indeed modified by TTLL4 in a cellular context. No clear consensus polyglutamylation site could be defined from the primary sequence of the here-identified new substrates of polyglutamylation. However, we demonstrate that glutamate-rich stretches are important for a protein to become polyglutamylated. Most of the newly identified substrates of polyglutamylation are nucleo-cytoplasmic shuttling proteins, including many chromatin binding proteins. Our work reveals that polyglutamylation is a much more widespread posttranslational modification than initially thought and thus that it might be a regulator of many cellular processes.
Fichier principal
Vignette du fichier
vanDijk_2JBC.pdf (962.61 Ko) Télécharger le fichier
Origine : Fichiers produits par l'(les) auteur(s)
Loading...

Dates et versions

hal-00217037 , version 1 (25-01-2008)

Identifiants

Citer

Juliette van Dijk, Julie Miro, Jean-Marc Strub, Benjamin Lacroix, Alain van Dorsselaer, et al.. Polyglutamylation is a posttranslational modification with a broad range of substrates.. Journal of Biological Chemistry, 2007, 283 (7), pp.3915-3922. ⟨10.1074/jbc.M705813200⟩. ⟨hal-00217037⟩
265 Consultations
202 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More