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Article Dans Une Revue European Journal of Immunology Année : 2008

Interleukin-10-induced MARCH1 mediates intracellular sequestration of MHC class II in monocytes.

Evelina Gatti

Résumé

IL-10 is a potent anti-inflammatory cytokine interfering with antigen presentation by inducing the intracellular sequestration of MHC class II (MHC-II) molecules. Here we studied the contribution of membrane-associated RING-CH (MARCH) ubiquitin ligase family members to the IL-10-induced down-regulation of MHC-II molecules. We found that MARCH1 and MARCH8 proteins are the most potent family members for the down-regulation of MHC-II surface expression in transfected cells, but only MARCH1 mRNA expression is strongly induced by IL-10 in human primary monocytes. We detected mono- and poly-ubiquitinated forms of MHC-II molecules both in IL-10-treated monocytes and in cells transfected with MARCH1. We also show direct interaction between MHC-II and MARCH1 molecules in co-immunoprecipitation assays. Finally, we found that siRNA-mediated knockdown of MARCH1 reverses IL-10-induced MHC-II down-regulation in primary monocytes. Thus, the immunosuppressive effect of IL-10 on antigen presentation is mediated through induced expression of MARCH1.

Domaines

Immunologie

Dates et versions

hal-00294112 , version 1 (08-07-2008)

Identifiants

Citer

Jacques Thibodeau, Marie-Claude Bourgeois-Daigneault, Gabrielle Huppé, Jessy Tremblay, Angélique Aumont, et al.. Interleukin-10-induced MARCH1 mediates intracellular sequestration of MHC class II in monocytes.. European Journal of Immunology, 2008, 38 (5), pp.1225-30. ⟨10.1002/eji.200737902⟩. ⟨hal-00294112⟩

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