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Article Dans Une Revue BBA - Biochimica et Biophysica Acta Année : 1988

Radioimmunofixation of human ferritin following serum isoelectric focusing.

Résumé

Radioimmunofixation of human ferritin following isoelectric focusing of serum was developed to study the microheterogeneity of this protein in native serum without previous purification or concentration. This method requires only 2-10 microliter of serum and can be used with levels of ferritin as low as 10 micrograms/l. In this way, the extensive microheterogeneity of this protein was revealed, since in some cases it produced as many as 35 bands with isoelectric points in a pH range of 4.95-5.9. Very different isoelectric focusing patterns (spectrotypes) of ferritin were observed during the investigation of pathological sera. The high sensitivity of this technique makes it useful for the investigation of serum ferritin in diseases involving modifications of the metabolism of this protein.Radioimmunofixation of human ferritin following isoelectric focusing of serum was developed to study the microheterogeneity of this protein in native serum without previous purification or concentration. This method requires only 2-10 microliter of serum and can be used with levels of ferritin as low as 10 micrograms/l. In this way, the extensive microheterogeneity of this protein was revealed, since in some cases it produced as many as 35 bands with isoelectric points in a pH range of 4.95-5.9. Very different isoelectric focusing patterns (spectrotypes) of ferritin were observed during the investigation of pathological sera. The high sensitivity of this technique makes it useful for the investigation of serum ferritin in diseases involving modifications of the metabolism of this protein.
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Dates et versions

hal-00313922 , version 1 (27-08-2008)

Identifiants

  • HAL Id : hal-00313922 , version 1

Citer

Y. Lasne, O. Benzerara, O. Damour, F. Lasne. Radioimmunofixation of human ferritin following serum isoelectric focusing.. BBA - Biochimica et Biophysica Acta, 1988, 964, pp.69-72. ⟨hal-00313922⟩
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