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Article Dans Une Revue Journal of Biomedical Optics Année : 2009

High heterogeneity of plasma membrane microfluidity in multidrug-resistant cancer cells

Résumé

Diffusion-time distribution analysis (DDA) has been used to explore the plasma membrane fluidity of multidrug-resistant cancer cells (LR73 carcinoma cells) and also to characterize the influence of various membrane agents present in the extracellular medium. DDA is a recent single-molecule technique, based on fluorescence correlation spectroscopy (FCS), well suited to retrieve local organization of cell membrane. The method was conducted on a large number of living cells, which enabled us to get a detailed overview of plasma membrane microviscosity, and plasma membrane micro-organization, between the cells of the same line. Thus, we clearly reveal the higher heterogeneity of plasma membrane in multidrug-resistant cancer cells in comparison with the nonresistant ones (denoted sensitive cells). We also display distinct modifications related to a membrane fluidity modulator, benzyl alcohol, and two revertants of multidrug resistance, verapamil and cyclosporin-A. A relation between the distribution of the diffusion-time values and the modification of membrane lateral heterogeneities is proposed

Dates et versions

hal-00415137 , version 1 (10-09-2009)

Identifiants

Citer

Céline Boutin, Yann Roche, Christine Millot, Régis Deturche, Pascal Royer, et al.. High heterogeneity of plasma membrane microfluidity in multidrug-resistant cancer cells. Journal of Biomedical Optics, 2009, 14 (3), pp.034030. ⟨10.1117/1.3155518⟩. ⟨hal-00415137⟩
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