Hepatocyte Growth Factor regulates SVZ cells proliferation in post-natal rodents - CNRS - Centre national de la recherche scientifique Accéder directement au contenu
Communication Dans Un Congrès Année : 2007

Hepatocyte Growth Factor regulates SVZ cells proliferation in post-natal rodents

Résumé

In the past decade, growing evidence has confirmed that neurogenesis occurs in the adult mammalian brain. New neurons are produced by neural stem cells (NSCs), many of which are located in the SVZ bordering the lateral ventricles. NSCs are of major interest for their potential therapeutical use in the treatment of neuropathological diseases. Yet, a better understanding of the mechanisms controlling proliferation and differentiation of SVZ cells is required before achieving clinical applications. Our study aims at identifying endogenous factors regulating the neurogenic activity of NSCs. We demonstrate that, in vitro, adult rat liver conditioned media increase SVZ proliferation, as determined by cell number counting and BrdU incorporation assay. Ultrafiltration assay provided evidence that the mitogenic effect is retained within the fraction with a relative molecular weight over 30 KDa. Among possible candidates released by the liver, we focused on Hepatocyte Growth Factor (HGF) because it is a known mitogene on various cell types. We found that exogenous HGF addition stimulates in a dose-dependent manner SVZ cells proliferation. Furthermore, the HGF receptor c-met was detected in SVZ cells by immunocytochemistry. Our ongoing studies will assess possible mitogenic effect of HGF in vivo and its functional role in brain neurogenic activity.
Fichier non déposé

Dates et versions

hal-00424102 , version 1 (14-10-2009)

Identifiants

  • HAL Id : hal-00424102 , version 1

Citer

Camille Nicoleau, Omar Benzakour, Fabienne Agasse, Laetitia Prestoz, Jérôme Petit, et al.. Hepatocyte Growth Factor regulates SVZ cells proliferation in post-natal rodents. Société des Neurosciences Francaises, Montpellier, 2007, Montpellier, France. pp.Abstract A10. ⟨hal-00424102⟩
83 Consultations
0 Téléchargements

Partager

Gmail Facebook X LinkedIn More