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Article Dans Une Revue Proceedings of the National Academy of Sciences of the United States of America Année : 2009

Crystallographic snapshots of iterative substrate translocations during nicotianamine synthesis in Archaea.

Résumé

Nicotianamine (NA), a small molecule ubiquitous in plants, is an important divalent metal chelator and the main precursor of phytosiderophores. Nicotianamine synthase (NAS) is the enzyme catalyzing NA synthesis by the condensation of three aminopropyl moieties of S-adenosylmethionine (SAM) and the cyclization of one of them to form an azetidine ring. Here we report five crystal structures of an archaeal NAS from Methanothermobacter thermautotrophicus, either free or in complex with its product(s) and substrate(s). These structures reveal a two-domains fold arrangement of MtNAS, a small molecule related to NA (named here thermoNicotianamine or tNA), and an original mechanism of synthesis in a buried reaction chamber. This reaction chamber is open to the solvent through a small inlet, and a single active site allows the selective entrance of only one substrate at a time that is then processed and translocated stepwise.
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Dates et versions

hal-00445313 , version 1 (31-05-2020)

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Cyril Dreyfus, David Lemaire, Stéphane Mari, David Pignol, Pascal Arnoux. Crystallographic snapshots of iterative substrate translocations during nicotianamine synthesis in Archaea.. Proceedings of the National Academy of Sciences of the United States of America, 2009, 106 (38), pp.16180-4. ⟨10.1073/pnas.0904439106⟩. ⟨hal-00445313⟩
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