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Article Dans Une Revue Journal of Biological Chemistry Année : 2010

Cell-penetrating peptides with intracellular actin-remodeling activity in malignant fibroblasts.

Résumé

Cell-penetrating peptides can cross cell membranes and are commonly seen as biologically inert molecules. However, we found that some cell-penetrating peptides could remodel actin cytoskeleton in oncogene-transformed NIH3T3/EWS-Fli cells. These cells have profound actin disorganization related to their tumoral transformation. These arginine- and/or tryptophan-rich peptides could cross cell membrane and induce stress fiber formation in these malignant cells, whereas they had no perceptible effect in non-tumoral fibroblasts. In addition, motility (migration speed, random motility coefficient, wound healing) of the tumor cells could be decreased by the cell-permeant peptides. Although the peptides differently influenced actin polymerization in vitro, they could directly bind monomeric actin as determined by NMR and calorimetry studies. Therefore, cell-penetrating peptides might interact with intracellular protein partners, such as actin. In addition, the fact that they could reverse the tumoral phenotype is of interest for therapeutic purposes.
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hal-00464266 , version 1 (31-05-2020)

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Diane Delaroche, François-Xavier Cantrelle, Frédéric Subra, Carine van Heijenoort, Eric Guittet, et al.. Cell-penetrating peptides with intracellular actin-remodeling activity in malignant fibroblasts.. Journal of Biological Chemistry, 2010, 285 (10), pp.7712-21. ⟨10.1074/jbc.M109.045872⟩. ⟨hal-00464266⟩
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