Vezatin, an integral membrane protein of adherens junctions, is required for the sound resilience of cochlear hair cells. - CNRS - Centre national de la recherche scientifique Accéder directement au contenu
Article Dans Une Revue EMBO Molecular Medicine Année : 2009

Vezatin, an integral membrane protein of adherens junctions, is required for the sound resilience of cochlear hair cells.

Amel Bahloul
Michel Leibovici
Paul Avan

Résumé

Loud sound exposure is a significant cause of hearing loss worldwide. We asked whether a lack of vezatin, an ubiquitous adherens junction protein, could result in noise-induced hearing loss. Conditional mutant mice bearing non-functional vezatin alleles only in the sensory cells of the inner ear (hair cells) indeed exhibited irreversible hearing loss after only one minute exposure to a 105 dB broadband sound. In addition, mutant mice spontaneously underwent late onset progressive hearing loss and vestibular dysfunction related to substantial hair cell death. We establish that vezatin is an integral membrane protein with two adjacent transmembrane domains, and cytoplasmic N- and C-terminal regions. Late recruitment of vezatin at junctions between MDCKII cells indicates that the protein does not play a role in the formation of junctions, but rather participates in their stability. Moreover, we show that vezatin directly interacts with radixin in its actin-binding conformation. Accordingly, we provide evidence that vezatin associates with actin filaments at cell-cell junctions. Our results emphasize the overlooked role of the junctions between hair cells and their supporting cells in the auditory epithelium resilience to sound trauma.

Dates et versions

hal-00477439 , version 1 (29-04-2010)

Identifiants

Citer

Amel Bahloul, Marie-Christine Simmler, Vincent Michel, Michel Leibovici, Isabelle Perfettini, et al.. Vezatin, an integral membrane protein of adherens junctions, is required for the sound resilience of cochlear hair cells.. EMBO Molecular Medicine, 2009, 1 (2), pp.125-38. ⟨10.1002/emmm.200900015⟩. ⟨hal-00477439⟩
352 Consultations
0 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More