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Article Dans Une Revue Nucleic Acids Research Année : 2010

The scaRNA2 is produced by an independent transcription unit and its processing is directed by the encoding region.

Résumé

The C/D box scaRNA2 is predicted to guide specific 2'-O-methylation of U2 snRNA. In contrast to other SCARNA genes, SCARNA2 appears to be independently transcribed. By transient expression of SCARNA2-reporter gene constructs, we have demonstrated that this gene is transcribed by RNA polymerase II and that the promoter elements responsible for its transcription are contained within a 161 bp region upstream of the transcription start site. In mammals, we have identified four cross species conserved promoter elements, a TATA motif, an hStaf/ZNF143 binding site and two novel elements that are required for full promoter activity. Binding of the human hStaf/ZNF143 transcription factor to its target sequence is required for promoter activity, suggesting that hStaf/ZNF143 is a fundamental regulator of the SCARNA2 gene. We also showed that RNA polymerase II continues transcription past the 3'-end of the mature RNA, irrespective of the identity of the Pol II promoter. The 3'-end processing and accumulation are governed by the sole information contained in the scaRNA2 encoding region, the maturation occurring via a particular pathway incompatible with that of mRNA or snRNA production.

Dates et versions

hal-00477441 , version 1 (29-04-2010)

Identifiants

Citer

Marie-Aline Gérard, Evelyne Myslinski, Natassia Chylak, Stéphanie Baudrey, Alain Krol, et al.. The scaRNA2 is produced by an independent transcription unit and its processing is directed by the encoding region.. Nucleic Acids Research, 2010, 38 (2), pp.370-81. ⟨10.1093/nar/gkp988⟩. ⟨hal-00477441⟩

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