High-chloride concentrations abolish the binding of adenine nucleotides in the mitochondrial ADP/ATP carrier family. - CNRS - Centre national de la recherche scientifique Accéder directement au contenu
Article Dans Une Revue Biophysical Journal Année : 2009

High-chloride concentrations abolish the binding of adenine nucleotides in the mitochondrial ADP/ATP carrier family.

Résumé

The ADP/ATP carrier (AAC) is a very effective membrane protein that mediates the exchange of ADP and ATP across the mitochondrial membrane. In vivo transport measurements on the AAC overexpressed in Escherichia coli demonstrate that this process can be severely inhibited by high-chloride concentrations. Molecular-dynamics simulations reveal a strong modification of the topology of the local electric field related to the number of chloride ions inside the cavity. Halide ions are shown to shield the positive charges lining the internal cavity of the carrier by accurate targeting of key basic residues. These specific amino acids are highly conserved as highlighted by the analysis of multiple AAC sequences. These results strongly suggest that the chloride concentration acts as an electrostatic lock for the mitochondrial AAC family, thereby preventing adenine nucleotides from reaching their dedicated binding sites.

Dates et versions

hal-00482875 , version 1 (11-05-2010)

Identifiants

Citer

Eva-Maria Krammer, Stéphanie Ravaud, François Dehez, Annie Frelet-Barrand, Eva Pebay-Peyroula, et al.. High-chloride concentrations abolish the binding of adenine nucleotides in the mitochondrial ADP/ATP carrier family.. Biophysical Journal, 2009, 97 (10), pp.L25-7. ⟨10.1016/j.bpj.2009.08.047⟩. ⟨hal-00482875⟩
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