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Article Dans Une Revue Cell Année : 2010

LINE-1 activity in facultative heterochromatin formation during X chromosome inactivation

Résumé

During X chromosome inactivation (XCI), Xist RNA coats and silences one of the two X chromosomes in female cells. Little is known about how XCI spreads across the chromosome, although LINE-1 elements have been proposed to play a role. Here we show that LINEs participate in creating a silent nuclear compartment into which genes become recruited. A subset of young LINE-1 elements, however, is expressed during XCI, rather than being silenced. We demonstrate that such LINE expression requires the specific heterochromatic state induced by Xist. These LINEs often lie within escape-prone regions of the X chromosome, but close to genes that are subject to XCI, and are associated with putative endo-siRNAs. LINEs may thus facilitate XCI at different levels, with silent LINEs participating in assembly of a heterochromatic nuclear compartment induced by Xist, and active LINEs participating in local propagation of XCI into regions that would otherwise be prone to escape.

Dates et versions

hal-00508558 , version 1 (04-08-2010)

Identifiants

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Jennifer Chow, Constance Ciaudo, Melissa Fazzari, Nathan Mise, Nicolas Servant, et al.. LINE-1 activity in facultative heterochromatin formation during X chromosome inactivation. Cell, 2010, 141 (6), pp.956-969. ⟨10.1016/j.cell.2010.04.042⟩. ⟨hal-00508558⟩
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