New design strategy for development of specific primer sets for PCR-based detection of Chlorophyceae and Bacillariophyceae in environmental samples. - CNRS - Centre national de la recherche scientifique Accéder directement au contenu
Article Dans Une Revue Applied and Environmental Microbiology Année : 2009

New design strategy for development of specific primer sets for PCR-based detection of Chlorophyceae and Bacillariophyceae in environmental samples.

Résumé

Studying aquatic microalgae is essential for monitoring biodiversity and water quality. We designed new sets of 18S rRNA PCR primers for Chlorophyceae and Bacillariophyceae by using the ARB software and implementing a virtual PCR program. The results of specificity analysis showed that most of the targeted algal families were identified and nontargeted organisms, such as fungi or ciliates, were excluded. These newly developed PCR primer sets were also able to amplify microalgal rRNA genes from environmental samples with accurate specificity. These tools could be of great interest for studying freshwater microalgal ecology and for developing bioindicators of the health status of aquatic environments.

Domaines

Ecotoxicologie

Dates et versions

hal-00528341 , version 1 (21-10-2010)

Identifiants

Citer

Claire Valiente Moro, Olivier Crouzet, Séréna Rasconi, Antoine Thouvenot, Gérard Coffe, et al.. New design strategy for development of specific primer sets for PCR-based detection of Chlorophyceae and Bacillariophyceae in environmental samples.. Applied and Environmental Microbiology, 2009, 75 (17), pp.5729-33. ⟨10.1128/AEM.00509-09⟩. ⟨hal-00528341⟩
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