Genome-wide evidence for an essential role of the human Staf/ZNF143 transcription factor in bidirectional transcription. - CNRS - Centre national de la recherche scientifique Accéder directement au contenu
Article Dans Une Revue Nucleic Acids Research Année : 2010

Genome-wide evidence for an essential role of the human Staf/ZNF143 transcription factor in bidirectional transcription.

Résumé

In the human genome, ∼10% of the genes are arranged head to head so that their transcription start sites reside within <1 kbp on opposite strands. In this configuration, a bidirectional promoter generally drives expression of the two genes. How bidirectional expression is performed from these particular promoters constitutes a puzzling question. Here, by a combination of in silico and biochemical approaches, we demonstrate that hStaf/ZNF143 is involved in controlling expression from a subset of divergent gene pairs. The binding sites for hStaf/ZNF143 (SBS) are overrepresented in bidirectional versus unidirectional promoters. Chromatin immunoprecipitation assays with a significant set of bidirectional promoters containing putative SBS revealed that 93% of them are associated with hStaf/ZNF143. Expression of dual reporter genes directed by bidirectional promoters are dependent on the SBS integrity and requires hStaf/ZNF143. Furthermore, in some cases, functional SBS are located in bidirectional promoters of gene pairs encoding a noncoding RNA and a protein gene. Remarkably, hStaf/ZNF143 per se exhibits an inherently bidirectional transcription activity, and together our data provide the demonstration that hStaf/ZNF143 is indeed a transcription factor controlling the expression of divergent protein-protein and protein-non-coding RNA gene pairs.

Dates et versions

hal-00561392 , version 1 (01-02-2011)

Identifiants

Citer

Yannick-Noël Anno, Evelyne Myslinski, Richard Patryk Ngondo-Mbongo, Alain Krol, Olivier Poch, et al.. Genome-wide evidence for an essential role of the human Staf/ZNF143 transcription factor in bidirectional transcription.. Nucleic Acids Research, 2010, epub ahead of print. ⟨10.1093/nar/gkq1301⟩. ⟨hal-00561392⟩
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