NLRP3 inflammasome is required in murine asthma in the absence of aluminum adjuvant. - CNRS - Centre national de la recherche scientifique Accéder directement au contenu
Article Dans Une Revue Allergy Année : 2011

NLRP3 inflammasome is required in murine asthma in the absence of aluminum adjuvant.

Résumé

To cite this article: Besnard A-G, Guillou N, Tschopp J, Erard F, Couillin I, Iwakura Y, Quesniaux V, Ryffel B, Togbe D. NLRP3 inflammasome is required in murine asthma in the absence of aluminum adjuvant. Allergy 2011; DOI: 10.1111/j.1398-9995.2011.02586.x. ABSTRACT: Background:  Inflammasome activation with the production of IL-1β received substantial attention recently in inflammatory diseases. However, the role of inflammasome in the pathogenesis of asthma is not clear. Using an adjuvant-free model of allergic lung inflammation induced by ovalbumin (OVA), we investigated the role of NLRP3 inflammasome and related it to IL-1R1 signaling pathway. Methods:  Allergic lung inflammation induced by OVA was evaluated in vivo in mice deficient in NLRP3 inflammasome, IL-1R1, IL-1β or IL-1α. Eosinophil recruitment, Th2 cytokine, and chemokine levels were determined in bronchoalveolar lavage fluid, lung homogenates, and mediastinal lymph node cells ex vivo. Results:  Allergic airway inflammation depends on NLRP3 inflammasome activation. Dendritic cell recruitment into lymph nodes, Th2 lymphocyte activation in the lung and secretion of Th2 cytokines and chemokines are reduced in the absence of NLRP3. Absence of NLRP3 and IL-1β is associated with reduced expression of other proinflammatory cytokines such as IL-5, IL-13, IL-33, and thymic stromal lymphopoietin. Furthermore, the critical role of IL-1R1 signaling in allergic inflammation is confirmed in IL-1R1-, IL-1β-, and IL-1α-deficient mice. Conclusion:  NLRP3 inflammasome activation leading to IL-1 production is critical for the induction of a Th2 inflammatory allergic response.

Domaines

Immunologie
Fichier non déposé

Dates et versions

hal-00591915 , version 1 (10-05-2011)

Identifiants

Citer

A.-G. Besnard, N. Guillou, J. Tschopp, François Erard, Isabelle Couillin, et al.. NLRP3 inflammasome is required in murine asthma in the absence of aluminum adjuvant.. Allergy, 2011, epub ahead of print. ⟨10.1111/j.1398-9995.2011.02586.x⟩. ⟨hal-00591915⟩

Collections

CNRS UNIV-ORLEANS
23 Consultations
0 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More