Stable-isotope labeling with amino acids in nematodes. - CNRS - Centre national de la recherche scientifique Accéder directement au contenu
Article Dans Une Revue Nature Methods Année : 2011

Stable-isotope labeling with amino acids in nematodes.

Résumé

We describe an approach for accurate quantitation of global protein dynamics in Caenorhabditis elegans. We adapted stable-isotope labeling with amino acids in cell culture (SILAC) for nematodes by feeding worms a heavy lysine- and heavy arginine-labeled Escherichia coli strain and report a genetic solution to elminate the problem of arginine-to-proline conversion. Combining our approach with quantitative proteomics methods, we characterized the heat-shock response in worms.

Dates et versions

hal-00649774 , version 1 (08-12-2011)

Identifiants

Citer

Mark Larance, Aymeric P Bailly, Ehsan Pourkarimi, Ronald T Hay, Grant Buchanan, et al.. Stable-isotope labeling with amino acids in nematodes.. Nature Methods, 2011, 8 (10), pp.849-51. ⟨10.1038/nmeth.1679⟩. ⟨hal-00649774⟩
24 Consultations
0 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More