BC1-FMRP interaction is modulated by 2'-O-methylation: RNA-binding activity of the tudor domain and translational regulation at synapses. - CNRS - Centre national de la recherche scientifique Accéder directement au contenu
Article Dans Une Revue Nucleic Acids Research Année : 2012

BC1-FMRP interaction is modulated by 2'-O-methylation: RNA-binding activity of the tudor domain and translational regulation at synapses.

Caroline Lacoux
  • Fonction : Auteur
Daniele Di Marino
  • Fonction : Auteur
Pietro Pilo Boyl
  • Fonction : Auteur
Francesca Zalfa
  • Fonction : Auteur
Bing Yan
  • Fonction : Auteur
Maria Teresa Ciotti
  • Fonction : Auteur
Mattia Falconi
  • Fonction : Auteur
Annie Mougin
  • Fonction : Auteur
  • PersonId : 837553
Claudia Bagni
  • Fonction : Auteur

Résumé

The brain cytoplasmic RNA, BC1, is a small non-coding RNA that is found in different RNP particles, some of which are involved in translational control. One component of BC1-containing RNP complexes is the fragile X mental retardation protein (FMRP) that is implicated in translational repression. Peptide mapping and computational simulations show that the tudor domain of FMRP makes specific contacts to BC1 RNA. Endogenous BC1 RNA is 2'-O-methylated in nucleotides that contact the FMRP interface, and methylation can affect this interaction. In the cell body BC1 2'-O-methylations are present in both the nucleus and the cytoplasm, but they are virtually absent at synapses where the FMRP-BC1-mRNA complex exerts its function. These results strongly suggest that subcellular region-specific modifications of BC1 affect the binding to FMRP and the interaction with its mRNA targets. We finally show that BC1 RNA has an important role in translation of certain mRNAs associated to FMRP. All together these findings provide further insights into the translational regulation by the FMRP-BC1 complex at synapses.

Dates et versions

hal-00667374 , version 1 (07-02-2012)

Identifiants

Citer

Caroline Lacoux, Daniele Di Marino, Pietro Pilo Boyl, Francesca Zalfa, Bing Yan, et al.. BC1-FMRP interaction is modulated by 2'-O-methylation: RNA-binding activity of the tudor domain and translational regulation at synapses.. Nucleic Acids Research, 2012, epub ahead of print. ⟨10.1093/nar/gkr1254⟩. ⟨hal-00667374⟩
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