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Article Dans Une Revue Curr Protoc Cell Biol Année : 2010

Purification of ribosomes from human cell lines.

Résumé

Highly conserved during evolution, the ribosome is the central effector of protein synthesis. In mammalian cells, the ribosome is a macromolecular complex composed of four different ribosomal RNAs (rRNA) and about 80 ribosomal proteins. Requiring more than 200 factors, ribosome biogenesis is a highly complex process that takes place mainly within the nucleoli of eukaryotic cells. Crystallographic data suggest that the ribosome is a ribozyme, in which the rRNA catalyses the peptide bond formation and ensures quality control of the translation. Ribosomal proteins are involved in this molecular mechanism; nonetheless, their role is still not fully characterized. Recent studies suggest that ribosomes themselves and/or the mechanisms underlying their synthesis, processing, and assembly play a key role in the establishment and progression of several human pathologies. The protocol described here is simple, efficient, and robust, and allows one to purify high-quality ribosomes from human cultured cell lines. Ribosomes purified with this protocol are adequate for most of the subsequent analyses of their RNA and protein content.
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Dates et versions

hal-00709414 , version 1 (18-06-2012)

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Stéphane Belin, Sabine Hacot, Lionel Daudignon, Gabriel Therizols, Stéphane Pourpe, et al.. Purification of ribosomes from human cell lines.. Curr Protoc Cell Biol, 2010, Chapter 3, pp.Unit 3.40. ⟨10.1002/0471143030.cb0340s49⟩. ⟨hal-00709414⟩
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