The Airway Microbiota in Cystic Fibrosis: A Complex Fungal and Bacterial Community-Implications for Therapeutic Management - CNRS - Centre national de la recherche scientifique Accéder directement au contenu
Article Dans Une Revue PLoS ONE Année : 2012

The Airway Microbiota in Cystic Fibrosis: A Complex Fungal and Bacterial Community-Implications for Therapeutic Management

Laurence Delhaes
  • Fonction : Auteur
Emilie Frealle
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Christine Hubans
  • Fonction : Auteur
Julia Salleron
Sylvie Leroy
  • Fonction : Auteur
A. Prevotat
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Frederick Wallet
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Benoît Wallaert
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Eduardo Dei-Cas
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  • PersonId : 917690

Résumé

Background Given the polymicrobial nature of pulmonary infections in patients with cystic fibrosis (CF), it is essential to enhance our knowledge on the composition of the microbial community to improve patient management. In this study, we developed a pyrosequencing approach to extensively explore the diversity and dynamics of fungal and prokaryotic populations in CF lower airways. Methodology and Principal Findings Fungi and bacteria diversity in eight sputum samples collected from four adult CF patients was investigated using conventional microbiological culturing and high-throughput pyrosequencing approach targeting the ITS2 locus and the 16S rDNA gene. The unveiled microbial community structure was compared to the clinical profile of the CF patients. Pyrosequencing confirmed recently reported bacterial diversity and observed complex fungal communities, in which more than 60% of the species or genera were not detected by cultures. Strikingly, the diversity and species richness of fungal and bacterial communities was significantly lower in patients with decreased lung function and poor clinical status. Values of Chao1 richness estimator were statistically correlated with values of the Shwachman-Kulczycki score, body mass index, forced vital capacity, and forced expiratory volume in 1 s (p = 0.046, 0.047, 0.004, and 0.001, respectively for fungal Chao1 indices, and p = 0.010, 0.047, 0.002, and 0.0003, respectively for bacterial Chao1 values). Phylogenetic analysis showed high molecular diversities at the sub-species level for the main fungal and bacterial taxa identified in the present study. Anaerobes were isolated with Pseudomonas aeruginosa, which was more likely to be observed in association with Candida albicans than with Aspergillus fumigatus.

Domaines

Océanographie

Dates et versions

hal-00806782 , version 1 (02-04-2013)

Identifiants

Citer

Laurence Delhaes, Sébastien Monchy, Emilie Frealle, Christine Hubans, Julia Salleron, et al.. The Airway Microbiota in Cystic Fibrosis: A Complex Fungal and Bacterial Community-Implications for Therapeutic Management. PLoS ONE, 2012, 7 (e36313), pp.4. ⟨10.1371/journal.pone.0036313⟩. ⟨hal-00806782⟩
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