Beclin-1 is required for chromosome congression and proper outer kinetochore assembly. - CNRS - Centre national de la recherche scientifique Accéder directement au contenu
Article Dans Une Revue EMBO Reports Année : 2013

Beclin-1 is required for chromosome congression and proper outer kinetochore assembly.

Marie Galloux
Katy Janvier
  • Fonction : Auteur
Roger E Karess
Clarisse Berlioz-Torrent

Résumé

The functions of Beclin-1 in macroautophagy, tumorigenesis and cytokinesis are thought to be mediated by its association with the PI3K-III complex. Here, we describe a new role for Beclin-1 in mitotic chromosome congression that is independent of the PI3K-III complex and its role in autophagy. Beclin-1 depletion in HeLa cells leads to a significant reduction of the outer kinetochore proteins CENP-E, CENP-F and ZW10, and, consequently, the cells present severe problems in chromosome congression. Beclin-1 associates with kinetochore microtubules and forms discrete foci near the kinetochores of attached chromosomes. We show that Beclin-1 interacts directly with Zwint-1-a component of the KMN (KNL-1/Mis12/Ndc80) complex-which is essential for kinetochore-microtubule interactions. This suggests that Beclin-1 acts downstream of the KMN complex to influence the recruitment of outer kinetochore proteins and promotes accurate kinetochore anchoring to the spindle during mitosis.

Dates et versions

hal-00813981 , version 1 (16-04-2013)

Identifiants

Citer

Stéphane Frémont, Annabelle Gérard, Marie Galloux, Katy Janvier, Roger E Karess, et al.. Beclin-1 is required for chromosome congression and proper outer kinetochore assembly.. EMBO Reports, 2013, 14 (4), pp.364-72. ⟨10.1038/embor.2013.23⟩. ⟨hal-00813981⟩
74 Consultations
0 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More