PiB-Conjugated, Metal-Based Imaging Probes: Multimodal Approaches for the Visualization of β-Amyloid Plaques - CNRS - Centre national de la recherche scientifique Accéder directement au contenu
Article Dans Une Revue Planetary and Space Science Année : 2013

PiB-Conjugated, Metal-Based Imaging Probes: Multimodal Approaches for the Visualization of β-Amyloid Plaques

Jean-François Morfin
Anna Kubícková
  • Fonction : Auteur
Vojtěch Kubícek
  • Fonction : Auteur
Frédéric Buron
Franck Suzenet
Milena Salerno
  • Fonction : Auteur
Adina N. Lazar
  • Fonction : Auteur
  • PersonId : 753927
  • IdHAL : adina-lazar
Charles Duyckaerts
  • Fonction : Auteur
Nicolas Arlicot
  • Fonction : Auteur
Éva Tóth

Résumé

In an effort toward the visualization of β-amyloid plaques by in vivo imaging techniques, we have conjugated an optimized derivative of the Pittsburgh compound B (PiB), a well-established marker of Aβ plaques, to DO3A-monoamide that is capable of forming stable, noncharged complexes with different trivalent metal ions including Gd3+ for MRI and 111In3+ for SPECT applications. Proton relaxivity measurements evidenced binding of Gd(DO3A-PiB) to the amyloid peptide Aβ1-40 and to human serum albumin, resulting in a two- and four-fold relaxivity increase, respectively. Ex vivo immunohistochemical studies showed that the DO3A-PiB complexes selectively target Aβ plaques on Alzheimer's disease human brain tissue. Ex vivo biodistribution data obtained for the 111In-analogue pointed to a moderate blood-brain barrier (BBB) penetration in adult male Swiss mice (without amyloid deposits) with 0.36% ID/g in the cortex at 2 min postinjection.

Dates et versions

hal-00840181 , version 1 (01-07-2013)

Identifiants

Citer

André F. Martins, Jean-François Morfin, Anna Kubícková, Vojtěch Kubícek, Frédéric Buron, et al.. PiB-Conjugated, Metal-Based Imaging Probes: Multimodal Approaches for the Visualization of β-Amyloid Plaques. Planetary and Space Science, 2013, 4 (5), pp.436-440. ⟨10.1021/ml400042w⟩. ⟨hal-00840181⟩
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