Long and short term intravital imaging reveals differential spatiotemporal recruitment and function of myelomonocytic cells after spinal cord injury. - CNRS - Centre national de la recherche scientifique Accéder directement au contenu
Article Dans Une Revue The Journal of Physiology Année : 2013

Long and short term intravital imaging reveals differential spatiotemporal recruitment and function of myelomonocytic cells after spinal cord injury.

Résumé

: After spinal cord injury (SCI) resident and peripheral myeloid cells are recruited to the injury site and play a role in injury progression. These cells are important for clearing cellular debris and can modulate the retraction and growth of axons in vitro. However, their precise spatiotemporal recruitment dynamics is unknown and their respective roles after SCI remain heavily debated. Using chronic, quantitative intravital two-photon microscopy of adult mice with SCI, here we show that infiltrating LysM(+) and resident CD11c(+) myelomonocytic cells have distinct spatiotemporal recruitment profiles and exhibit changes in morphology, motility, phagocytic activity, and axon interaction patterns over time. This study provides the first in vivo description of the influx of inflammatory and resident myelomonocytic cells into the injured spinal cord and their interactions with cut axons and underscores the importance of precise timing and targeting of specific cell populations in developing therapies for SCI.

Dates et versions

hal-00862129 , version 1 (16-09-2013)

Identifiants

Citer

Keith K Fenrich, Pascal Weber, Geneviève Rougon, Franck Debarbieux. Long and short term intravital imaging reveals differential spatiotemporal recruitment and function of myelomonocytic cells after spinal cord injury.. The Journal of Physiology, 2013, epub ahead of print. ⟨10.1113/jphysiol.2013.256388⟩. ⟨hal-00862129⟩

Collections

CNRS UNIV-AMU IBDM
23 Consultations
0 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More