Citric acid-γ-cyclodextrin crosslinked oligomers as carriers for doxorubicin delivery - CNRS - Centre national de la recherche scientifique Accéder directement au contenu
Article Dans Une Revue Photochemical & Photobiological Sciences Année : 2013

Citric acid-γ-cyclodextrin crosslinked oligomers as carriers for doxorubicin delivery

Resmi Anand
  • Fonction : Auteur
Ilse Manet
  • Fonction : Auteur
Francesco Manoli
  • Fonction : Auteur
Ahmet Aykaç
  • Fonction : Auteur
Antonio Vargas-Berenguel
  • Fonction : Auteur
Ruxandra Gref
Sandra Monti
  • Fonction : Auteur

Résumé

Two citric acid crosslinked γ-cyclodextrin oligomers (pγ-CyD) with a MW of 21-33 kDa and 10-15 γ-CyD units per molecule were prepared by following green chemistry methods and were fully characterized. The non-covalent association of doxorubicin (DOX) with these macromolecules was investigated in neutral aqueous medium by means of circular dichroism (CD), UV-vis absorption and fluorescence. Global analysis of multiwavelength spectroscopic CD and fluorescence titration data, taking into account the DOX monomer-dimer equilibrium, evidenced the formation of 1 : 1 and 1 : 2 pγ-CyD unit-DOX complexes. The binding constants are 1-2 orders of magnitude higher than those obtained for γ-CyD and depend on the characteristics of the oligomer batch used. The concentration profiles of the species in solution evidence the progressive monomerization of DOX with increasing oligomer concentration. Confocal fluorescence imaging and spectral imaging showed a similar drug distribution within the MCF-7 cell line incubated with either DOX complexed to pγ-CyD or free DOX. In both cases DOX is taken up into the cell nucleus without any degradation.

Dates et versions

hal-00869828 , version 1 (04-10-2013)

Identifiants

Citer

Resmi Anand, Milo Malanga, Ilse Manet, Francesco Manoli, Kata Tuza, et al.. Citric acid-γ-cyclodextrin crosslinked oligomers as carriers for doxorubicin delivery. Photochemical & Photobiological Sciences , 2013, 12, pp.1841-1854. ⟨10.1039/C3PP50169H⟩. ⟨hal-00869828⟩
43 Consultations
0 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More