Three different classes of aminotransferases evolved prephenate aminotransferase functionality in arogenate-competent microorganisms. - CNRS - Centre national de la recherche scientifique Accéder directement au contenu
Article Dans Une Revue Journal of Biological Chemistry Année : 2014

Three different classes of aminotransferases evolved prephenate aminotransferase functionality in arogenate-competent microorganisms.

Résumé

The aromatic amino acids phenylalanine and tyrosine represent essential sources of high value natural aromatic compounds for human health and industry. Depending on the organism, alternative routes exist for their synthesis. Phenylalanine and tyrosine are synthesized either via phenylpyruvate/4-hydroxyphenylpyruvate or via arogenate. In arogenate-competent microorganisms, an aminotransferase is required for the transamination of prephenate into arogenate, but the identity of the genes is still unknown. We present here the first identification of prephenate aminotransferases (PATs) in seven arogenate-competent microorganisms and the discovery that PAT activity is provided by three different classes of aminotransferase, which belong to two different fold types of pyridoxal phosphate enzymes: an aspartate aminotransferase subgroup 1β in tested α- and β-proteobacteria, a branched-chain aminotransferase in tested cyanobacteria, and an N-succinyldiaminopimelate aminotransferase in tested actinobacteria and in the β-proteobacterium Nitrosomonas europaea. Recombinant PAT enzymes exhibit high activity toward prephenate, indicating that the corresponding genes encode bona fide PAT. PAT functionality was acquired without other modification of substrate specificity and is not a general catalytic property of the three classes of aminotransferases.
Fichier principal
Vignette du fichier
2014_Graindorge_Journal of Biological Chemistry_1.pdf (2.9 Mo) Télécharger le fichier
Origine : Fichiers éditeurs autorisés sur une archive ouverte
Loading...

Dates et versions

hal-00943539 , version 1 (28-05-2020)

Licence

Copyright (Tous droits réservés)

Identifiants

Citer

Matthieu Graindorge, Cécile Giustini, Alexandra Kraut, Lucas Moyet, Gilles Curien, et al.. Three different classes of aminotransferases evolved prephenate aminotransferase functionality in arogenate-competent microorganisms.. Journal of Biological Chemistry, 2014, 289 (6), pp.3198-208. ⟨10.1074/jbc.M113.486480⟩. ⟨hal-00943539⟩
113 Consultations
27 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More