Electrochemical Detection of Single Microbeads Manipulated by Optical Tweezers in the Vicinity of Ultramicroelectrodes - CNRS - Centre national de la recherche scientifique Accéder directement au contenu
Article Dans Une Revue Analytical Chemistry Année : 2013

Electrochemical Detection of Single Microbeads Manipulated by Optical Tweezers in the Vicinity of Ultramicroelectrodes

Résumé

Latex micrometric beads are manipulated by optical tweezers in the vicinity of an ultramicroelectrode (UME). They are optically trapped in solution and approached the electrode surface. After the electrochemical measurement, they are optically removed from the surface. The residence time of the particle on the electrode is thus controlled by the optical tweezers. The detection is based on diffusional hindrance by the insulating objects which alters the fluxes of the redox Ru(NH3)6 3+ species toward the UME and thus its mass-transfer limited current. We have optically deposited successively 1, 2, and 3 beads of 3-μm radius on the UME surface, and we have recorded the variations of the current depending on their landing locations that were optically controlled. Finally we decreased the current by partially blocking the electroactive surface with a six-bead assembly. The variation of the steady-state current and the approach curves allow for the indirect electrochemical localization of the bead in the vicinity of the UME, not only when the bead is in contact but also when it is levitated at distances lower than the UME radius. ....
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Dates et versions

hal-00989615 , version 1 (12-05-2014)

Identifiants

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Emmanuel Suraniti, Frédéric Kanoufi, Charlie Gosse, Xuan Zhao, Rumiana Dimova, et al.. Electrochemical Detection of Single Microbeads Manipulated by Optical Tweezers in the Vicinity of Ultramicroelectrodes. Analytical Chemistry, 2013, 85, pp. 8902−8909. ⟨10.1021/ac402200p⟩. ⟨hal-00989615⟩
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