Hybrid polarizing solids for pure hyperpolarized liquids through dissolution dynamic nuclear polarization. - CNRS - Centre national de la recherche scientifique Accéder directement au contenu
Article Dans Une Revue Proceedings of the National Academy of Sciences of the United States of America Année : 2014

Hybrid polarizing solids for pure hyperpolarized liquids through dissolution dynamic nuclear polarization.

Aurélien Bornet
  • Fonction : Auteur
Basile Vuichoud
  • Fonction : Auteur
Jonas Milani
  • Fonction : Auteur
Roberto Melzi
  • Fonction : Auteur
Henri A van Kalkeren
  • Fonction : Auteur

Résumé

: Hyperpolarization of substrates for magnetic resonance spectroscopy (MRS) and imaging (MRI) by dissolution dynamic nuclear polarization (D-DNP) usually involves saturating the ESR transitions of polarizing agents (PAs; e.g., persistent radicals embedded in frozen glassy matrices). This approach has shown enormous potential to achieve greatly enhanced nuclear spin polarization, but the presence of PAs and/or glassing agents in the sample after dissolution can raise concerns for in vivo MRI applications, such as perturbing molecular interactions, and may induce the erosion of hyperpolarization in spectroscopy and MRI. We show that D-DNP can be performed efficiently with hybrid polarizing solids (HYPSOs) with 2,2,6,6-tetramethyl-piperidine-1-oxyl radicals incorporated in a mesostructured silica material and homogeneously distributed along its pore channels. The powder is wetted with a solution containing molecules of interest (for example, metabolites for MRS or MRI) to fill the pore channels (incipient wetness impregnation), and DNP is performed at low temperatures in a very efficient manner. This approach allows high polarization without the need for glass-forming agents and is applicable to a broad range of substrates, including peptides and metabolites. During dissolution, HYPSO is physically retained by simple filtration in the cryostat of the DNP polarizer, and a pure hyperpolarized solution is collected within a few seconds. The resulting solution contains the pure substrate, is free from any paramagnetic or other pollutants, and is ready for in vivo infusion.

Domaines

Chimie organique

Dates et versions

hal-01070302 , version 1 (01-10-2014)

Identifiants

Citer

David Gajan, Aurélien Bornet, Basile Vuichoud, Jonas Milani, Roberto Melzi, et al.. Hybrid polarizing solids for pure hyperpolarized liquids through dissolution dynamic nuclear polarization.. Proceedings of the National Academy of Sciences of the United States of America, 2014, 111 (41), pp.14693-14697. ⟨10.1073/pnas.1407730111⟩. ⟨hal-01070302⟩
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