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Article Dans Une Revue Cell Reports Année : 2014

A Simple Method for 3D Analysis of Immunolabeled Axonal Tracts in a Transparent Nervous System

Résumé

Graphical Abstract Highlights Immunostaining and 3DISCO clearing: a powerful method for studying brain connections 3D analysis of axon guidance defects in midline mutant mice Unexpected roles for Slits and Netrin-1 in fasciculus retroflexus development In Brief Clearing techniques have recently been developed to look at mouse brains, but they are complex and expensive. Belle et al. now describe a simple procedure that combines immunolabeling, solvent-based clearing, and light-sheet fluores-cence microscopy. This technique allows large-scale screening of axon guidance defects and other developmental disor-ders in mutant mice.
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Dates et versions

hal-01083957 , version 1 (18-11-2014)

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Morgane Belle, David Godefroy, Chloé Dominici, Céline Heitz-Marchaland, Pavol Zelina, et al.. A Simple Method for 3D Analysis of Immunolabeled Axonal Tracts in a Transparent Nervous System. Cell Reports, 2014, 9 (4), pp.1191-1201. ⟨10.1016/j.celrep.2014.10.037⟩. ⟨hal-01083957⟩
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