Interaction between the elastin peptide VGVAPG and human elastin binding protein. - CNRS - Centre national de la recherche scientifique Accéder directement au contenu
Article Dans Une Revue Journal of Biological Chemistry Année : 2013

Interaction between the elastin peptide VGVAPG and human elastin binding protein.

Amandine Scandolera
  • Fonction : Auteur
Stéphanie Baud

Résumé

The elastin binding protein (EBP), a spliced variant of lysosomal β-galactosidase, is the primary receptor of elastin peptides that have been linked to emphysema, aneurysm and cancer progression. The sequences recognized by EBP share the XGXXPG consensus pattern found in numerous matrix proteins, notably in elastin where the VGVAPG motif is repeated. To delineate the elastin binding site of human EBP, we built a homology model of this protein and docked VGVAPG on its surface. Analysis of this model suggested that Gln-97 and Asp-98 were required for interaction with VGVAPG because they contribute to the definition of a pocket thought to represent the elastin binding site of EBP. Additionally, we proposed that Leu-103, Arg-107, and Glu-137 were essential residues because they could interact with VGVAPG itself. Site-directed mutagenesis experiments at these key positions validated our model. This work therefore provides the first structural data concerning the interaction of the VGVAPG with its cognate receptor. The present structural data should now allow the development of EBP-specific antagonists.

Domaines

Chimie organique
Fichier principal
Vignette du fichier
1-s2.0-S002192582046675X-main.pdf (2.53 Mo) Télécharger le fichier
Origine : Fichiers éditeurs autorisés sur une archive ouverte

Dates et versions

hal-01109325 , version 1 (27-05-2021)

Licence

Paternité

Identifiants

Citer

Charlotte Blanchevoye, Nicolas Floquet, Amandine Scandolera, Stéphanie Baud, Pascal Maurice, et al.. Interaction between the elastin peptide VGVAPG and human elastin binding protein.. Journal of Biological Chemistry, 2013, 288 (2), pp.1317-1328. ⟨10.1074/jbc.M112.419929⟩. ⟨hal-01109325⟩
246 Consultations
52 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More