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Article Dans Une Revue Acta crystallographica Section D : Structural biology [1993-...] Année : 2014

Weak protein–cationic co-ion interactions addressed by X-ray crystallography and mass spectrometry

Résumé

The adsorption of Rb+, Cs+, Mn2+, Co2+ and Yb3+ onto the positively charged hen egg-white lysozyme (HEWL) has been investigated by solving 13 X-ray structures of HEWL crystallized with their chlorides and by applying electrospray ionization mass spectrometry (ESI-MS) first to dissolved protein crystals and then to the protein in buffered salt solutions. The number of bound cations follows the order Cs+ < Mn2+ ’ Co2+ < Yb3+ at 293 K. HEWL binds less Rb+ (qtot = 0.7) than Cs+ (qtot = 3.9) at 100 K. Crystal flash-cooling drastically increases the binding of Cs+, but poorly affects that of Yb3+, suggesting different interactions. The addition of glycerol increases the number of bound Yb3+ cations, but only slightly increases that of Rb+. HEWL titrations with the same chlorides, followed by ESI-MS analysis, show that only about 10% of HEWL binds Cs+ and about 40% binds 1–2 Yb3+ cations, while the highest binding reaches 60–70% for protein binding 1–3 Mn2+ or Co2+ cations. The binding sites identified by X-ray crystallography show that the monovalent Rb+ and Cs+ preferentially bind to carbonyl groups, whereas the multivalent Mn2+, Co2+ and Yb3+ interact with carboxylic groups. This work elucidates the basis of the effect of the Hofmeister cation series on protein solubility.
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hal-01120260 , version 1 (02-03-2015)

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Philippe Bénas, Nicolas Auzeil, Laurent Legrand, Franck Brachet, Anne Regazzetti, et al.. Weak protein–cationic co-ion interactions addressed by X-ray crystallography and mass spectrometry. Acta crystallographica Section D : Structural biology [1993-..], 2014, 70 (8), pp.2217-2231. ⟨10.1107/S1399004714011304⟩. ⟨hal-01120260⟩
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