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Article Dans Une Revue Molecular Ecology Resources Année : 2015

OBITools: a Unix-inspired software package for DNA metabarcoding

Frédéric Boyer
Aurélie Bonin
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Yvan Le Bras
Pierre Taberlet
Eric Coissac

Résumé

DNA metabarcoding offers new perspectives in biodiversity research. This recently developed approach to ecosystem study relies heavily on the use of next-generation sequencing (NGS) and thus calls upon the ability to deal with huge sequence data sets. The obitools package satisfies this requirement thanks to a set of programs specifically designed for analysing NGS data in a DNA metabarcoding context. Their capacity to filter and edit sequences while taking into account taxonomic annotation helps to set up tailor-made analysis pipelines for a broad range of DNA metabarcoding applications, including biodiversity surveys or diet analyses. The obitools package is distributed as an open source software available on the following website: http://metabarcoding.org/obitools. A Galaxy wrapper is available on the GenOuest core facility toolshed: http://toolshed.genouest.org.

Dates et versions

hal-01183582 , version 1 (10-08-2015)

Identifiants

Citer

Frédéric Boyer, Céline Mercier, Aurélie Bonin, Yvan Le Bras, Pierre Taberlet, et al.. OBITools: a Unix-inspired software package for DNA metabarcoding. Molecular Ecology Resources, 2015, pp.10.1111/1755-0998.12428. ⟨10.1111/1755-0998.12428⟩. ⟨hal-01183582⟩
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