Biochemical characterization of glutaredoxins from Chlamydomonas reinhardtii: kinetics and specificity in deglutathionylation reactions. - CNRS - Centre national de la recherche scientifique Accéder directement au contenu
Article Dans Une Revue FEBS Letters Année : 2010

Biochemical characterization of glutaredoxins from Chlamydomonas reinhardtii: kinetics and specificity in deglutathionylation reactions.

Résumé

Protein deglutathionylation is mainly catalyzed by glutaredoxins (GRXs). We have analyzed the biochemical properties of four of the six different GRXs of Chlamydomonas reinhardtii. Kinetic parameters were determined for disulfide and dehydroascorbate reduction but also for deglutathionylation of artificial and protein substrates. The results indicate that GRXs exhibit striking differences in their catalytic properties, mainly linked to the class of GRX considered but also to the pK(a) of the N-terminal catalytic cysteine. Furthermore, glutathionylated proteins were found to exhibit distinct reactivities with GRXs. These results suggest that glutathionylation may allow a fine tuning of cell metabolism under stress conditions.

Dates et versions

hal-01183612 , version 1 (10-08-2015)

Identifiants

Citer

Xing-Huang Gao, Mirko Zaffagnini, Mariette Bedhomme, Laure Michelet, Corinne Cassier-Chauvat, et al.. Biochemical characterization of glutaredoxins from Chlamydomonas reinhardtii: kinetics and specificity in deglutathionylation reactions.. FEBS Letters, 2010, 584 (11), pp.2242-8. ⟨10.1016/j.febslet.2010.04.034⟩. ⟨hal-01183612⟩
98 Consultations
0 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More