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Poster De Conférence Année : 2014

Milk sphingomyelin domains in biomimetic membranes and the role of cholesterol - investigation using AFM and force spectroscopy

Résumé

Milk sphingomyelin (MSM) and cholesterol segregate into domains in the outer bilayer membrane surrounding milk fat globules. To elucidate the morphology and mechanical properties of theses domains, supported lipid bilayers with controlled molar proportions of MSM, dioleoylphosphatidylcholine (DOPC) and cholesterol were produced in buffer mimicking conditions of the milk aqueous phase. Atomic force microscopy imaging showed that (i) for T <35 ° C MSM segregated in gel phase domains protruding above the fluid phase, (ii) the addition of 20 mol % cholesterol resulted in smaller and more elongated lophase domains than in equimolar MSM/DOPC membranes, (iii) the MSM/cholesterol-enrichedl o phase domains were less salient than the MSM gel phase domains. Force spectroscopy measurements furthermore showed that cholesterol reduced the resistance of MSM/DOPC membrane to perforation. The results are discussed with respect to the effect of cholesterol on the biophysical properties of lipid membranes. The combination of AFM imaging and force mapping provides unprecedented insight into the structural and mechanical properties of milk lipid membranes, and opens perspectives for investigation of the functional properties of MSM domains during milk fat processing or digestion
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Dates et versions

hal-01209638 , version 1 (02-10-2015)

Identifiants

  • HAL Id : hal-01209638 , version 1
  • PRODINRA : 262537

Citer

Fanny Guyomarc'H, Shan Zou, Maohui Chen, Pierre-Emmanuel Milhiet, Cedric Godefroy, et al.. Milk sphingomyelin domains in biomimetic membranes and the role of cholesterol - investigation using AFM and force spectroscopy. 6.Rencontres de Biologie Physique du Grand Ouest, Jun 2014, le Mans, France. , 2014. ⟨hal-01209638⟩
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