glnD and mviN are genes of an essential operon in Sinorhizobium meliloti
Résumé
To evaluate the role of uridylyl-transferase, the Sinorhizobium meliloti glnD gene was isolated by heterologous complementation in Azotobacter vinelandii. The glnD gene is cotranscribed with a gene homologous to Salmonella mviN. glnD1::Omega or mviN1::Omega mutants could not be isolated by a powerful sucrose counterselection procedure unless a complementing cosmid was provided, indicating that glnD and mviN are members of an indispensable operon in S. meliloti.
Domaines
Protistologie
Fichier principal
2001_Rudnick_J Bacteriol_{830A8F48-1122-4463-BDB2-BA3A026BF123}.pdf (224.05 Ko)
Télécharger le fichier
Origine : Accord explicite pour ce dépôt
Loading...