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Article Dans Une Revue Methods in Molecular Biology Année : 2014

Sampling and Isolation of C. elegans from the Natural Habitat.

Nausicaa Poullet
Christian Braendle

Résumé

Wild populations of the model organism C. elegans allow characterization of natural genetic variation underlying diverse phenotypic traits. Here we provide a simple protocol on how to sample and rapidly identify C. elegans wild isolates. We outline how to find suitable habitats and organic substrates, followed by describing isolation and identification of C. elegans live cultures based on easily recognizable morphological characteristics, molecular barcodes and/or mating tests. This protocol uses standard laboratory equipment and requires no prior knowledge of C. elegans biology.
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hal-01252948 , version 1 (08-01-2016)

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  • HAL Id : hal-01252948 , version 1
  • PUBMED : 26423978

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Nausicaa Poullet, Christian Braendle. Sampling and Isolation of C. elegans from the Natural Habitat.. Methods in Molecular Biology, 2014, 1327, pp.221-9. ⟨hal-01252948⟩
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