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Article Dans Une Revue Tetrahedron Letters Année : 2013

A substrate for the detection of broad specificity alpha-L-arabinofuranosidases with indirect release of a chromogenic group

Résumé

The synthesis of a compound containing a 4-nitrocatechol bound to two vicinal alpha-L-arabinofuranosyl moieties through a linker arm was achieved using a sulfate protecting group to facilitate selective alkylation of one aromatic hydroxyl. Several alpha-L-arabinofuranosidases displaying different selectivities were tested and a simple microtiter plate-based assay was developed. The observed resistance of the compound to alpha-L-arabinofuranosidase-mediated hydrolysis makes it suitable for the identification of enzymes that are able to accommodate bis-arabinofuranosylated moieties. (C) 2013 Elsevier Ltd. All rights reserved.
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Dates et versions

hal-01268100 , version 1 (04-02-2016)

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Vinciane Borsenberger, Emmie Dornez, Marie-Laure Desrousseaux, Christophe M. Courtin, Michael O'Donohue, et al.. A substrate for the detection of broad specificity alpha-L-arabinofuranosidases with indirect release of a chromogenic group. Tetrahedron Letters, 2013, 54 (24), pp.3063 - 3066. ⟨10.1016/j.tetlet.2013.03.136⟩. ⟨hal-01268100⟩
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