Characterization of Glycosyltransferase Activity of Wild-Type Leuconostoc mesenteroides Strains from Bulgarian Fermented Vegetables - CNRS - Centre national de la recherche scientifique Accéder directement au contenu
Article Dans Une Revue Applied Biochemistry and Biotechnology Année : 2012

Characterization of Glycosyltransferase Activity of Wild-Type Leuconostoc mesenteroides Strains from Bulgarian Fermented Vegetables

Résumé

Glycosyltransferase activity of 13 Leuconostoc mesenteroides strains isolated from Bulgarian fermented vegetables was investigated. All the strains displayed a mucoid phenotype on sucrose-containing agar media. Strains were characterized according to carbohydrate fermentation, species-specific multiple PCR using several primers, repetitive element-PCR fingerprinting using (GTG)(5) primers and glycosyltransferase activity. Level of activity and cellular localization (soluble or cell-associated) were variable among strains. Precipitation of exopolysaccharides produced from sucrose by the soluble fractions from these strains allowed recovery of only glucans and further characterization by H-1 and C-13 NMR analysis and enzymatic digestion with dextranase revealed dextran production. However, levans could be detected in presence of raffinose as fructosyl donor. Both fructosyltransferase and glucosyltransferase encoding genes were detected by PCR and both active enzymes were detected after functional characterization by SDS-PAGE electrophoresis and in situ polymer production after incubation with sucrose. This work therefore showed that concomitant production of glucosyltransferase and fructosyltransferase is widespread in L. mesenteroides strains.

Dates et versions

hal-01268287 , version 1 (04-02-2016)

Identifiants

Citer

Tonka Vasileva, Ilia Iliev, Myriam Amari, Veselin Bivolarski, Marie-Sophie Bounaix, et al.. Characterization of Glycosyltransferase Activity of Wild-Type Leuconostoc mesenteroides Strains from Bulgarian Fermented Vegetables. Applied Biochemistry and Biotechnology, 2012, 168 (3), pp.718 - 730. ⟨10.1007/s12010-012-9812-7⟩. ⟨hal-01268287⟩
57 Consultations
0 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More