Persistence of detectable insecticidal proteins from [i]Bacillus thuringiensis[/i] (Cry) and toxicity after adsorption on contrasting soils - CNRS - Centre national de la recherche scientifique Accéder directement au contenu
Article Dans Une Revue Environmental Pollution Année : 2016

Persistence of detectable insecticidal proteins from [i]Bacillus thuringiensis[/i] (Cry) and toxicity after adsorption on contrasting soils

Résumé

Insecticidal Cry, or Bt, proteins are produced by the soil-endemic bacterium, Bacillus thuringiensis and some genetically modified crops. Their environmental fate depends on interactions with soil. Little is known about the toxicity of adsorbed proteins and the change in toxicity over time. We incubated Cry1Ac and Cry2A in contrasting soils subjected to different treatments to inhibit microbial activity. The toxin was chemically extracted and immunoassayed. Manduca sexta was the target insect for biotests. Extractable toxin decreased during incubation for up to four weeks. Toxicity of Cry1Ac was maintained in the adsorbed state, but lost after 2 weeks incubation at 25 °C. The decline in extractable protein and toxicity were much slower at 4 °C with no significant effect of soil sterilization. The major driving force for decline may be time-dependent fixation of adsorbed protein, leading to a decrease in the extraction yield in vitro, paralleled by decreasing solubilisation in the larval gut.

Domaines

Géochimie
Fichier non déposé

Dates et versions

hal-01269359 , version 1 (05-02-2016)

Identifiants

Citer

Hung Truong Phuc, Truong Le Van, N. D. Binh, Roger Frutos, Herve Quiquampoix, et al.. Persistence of detectable insecticidal proteins from [i]Bacillus thuringiensis[/i] (Cry) and toxicity after adsorption on contrasting soils. Environmental Pollution, 2016, 208, pp.318-325. ⟨10.1016/j.envpol.2015.09.046⟩. ⟨hal-01269359⟩
153 Consultations
0 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More