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Article Dans Une Revue BBA - Gene Structure and Expression Année : 2002

Molecular cloning of chicken prepro-orexin cDNA and preferential expression in the chicken hypothalamus

Résumé

Chicken prepro-orexin cDNA has been cloned, sequenced and characterized. The predicted amino acid sequence of chicken prepro-orexin cDNA revealed that orexin-A and -B are highly conserved among vertebrate species. In situ hybridization and immunohistochemistry localized orexin-positive cell bodies in the periventricular hypothalamic nucleus extending into the lateral hypothalamic area. Comparisons of orexin gene expression in the brains of 24-h-fasted and ad libitum-fed chickens were made using semi-quantitative RT-PCR. No significant differences in orexin mRNA expression were observed.

Dates et versions

hal-01308878 , version 1 (28-04-2016)

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Takeshi Ohkubo, Tim Boswell, Sophie Lumineau. Molecular cloning of chicken prepro-orexin cDNA and preferential expression in the chicken hypothalamus. BBA - Gene Structure and Expression, 2002, 1577 (3), pp.476--480. ⟨10.1016/S0167-4781(02)00483-9⟩. ⟨hal-01308878⟩
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