Tma108, a putative M1 aminopeptidase, is a specific nascent chain-associated protein in Saccharomyces cerevisiae - CNRS - Centre national de la recherche scientifique Accéder directement au contenu
Article Dans Une Revue Nucleic Acids Research Année : 2016

Tma108, a putative M1 aminopeptidase, is a specific nascent chain-associated protein in Saccharomyces cerevisiae

Thibaut Leger
Gaëlle Lelandais
  • Fonction : Auteur
  • PersonId : 879919
Benoit Palancade

Résumé

The discovery of novel specific ribosome-associated factors challenges the assumption that translation relies on standardized molecular machinery. In this work, we demonstrate that Tma108, an uncharac-terized translation machinery-associated factor in yeast, defines a subpopulation of cellular ribosomes specifically involved in the translation of less than 200 mRNAs encoding proteins with ATP or Zinc binding domains. Using ribonucleoparticle dissoci-ation experiments we established that Tma108 directly interacts with the nascent protein chain. Additionally , we have shown that translation of the first 35 amino acids of Asn1, one of the Tma108 targets, is necessary and sufficient to recruit Tma108, suggesting that it is loaded early during translation. Comparative genomic analyses, molecular model-ing and directed mutagenesis point to Tma108 as an original M1 metallopeptidase, which uses its pu-tative catalytic peptide-binding pocket to bind the N-terminus of its targets. The involvement of Tma108 in co-translational regulation is attested by a drastic change in the subcellular localization of ATP2 mRNA upon Tma108 inactivation. Tma108 is a unique example of a nascent chain-associated factor with high selectivity and its study illustrates the existence of other specific translation-associated factors besides RNA binding proteins.
Fichier principal
Vignette du fichier
Nucl. Acids Res.-2016-Delaveau-nar_gkw732.pdf (3.35 Mo) Télécharger le fichier
Origine : Publication financée par une institution
Loading...

Dates et versions

hal-01361729 , version 1 (07-09-2016)

Licence

Paternité - Pas d'utilisation commerciale

Identifiants

Citer

Thierry Delaveau, Dimitri Davoine, Ariane Jolly, Antoine Vallot, Jérôme O. Rouvière, et al.. Tma108, a putative M1 aminopeptidase, is a specific nascent chain-associated protein in Saccharomyces cerevisiae. Nucleic Acids Research, 2016, 44 (18), pp.8826-8841. ⟨10.1093/nar/gkw732⟩. ⟨hal-01361729⟩
585 Consultations
132 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More