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Article Dans Une Revue Journal of Physical Chemistry Letters Année : 2014

Toward Quantitative Measurements of Enzyme Kinetics by Dissolution Dynamic Nuclear Polarization

Résumé

Dissolution dynamic nuclear polarization (D-DNP) experiments enabled us to study the kinetics of the enzymatic phosphorylation reaction of glucose to form glucose-6-phosphate (G6P) by hexokinase (HK), with or without the presence of an excess of G6P, which is known to be an inhibitor of the enzyme. Against all expectations, our observations demonstrate that the phosphorylation of both alpha and beta glucose anomers occurs with comparable kinetics. The catalytic constant of the reaction was estimated based on a simple kinetic model tailored for hyperpolarized systems.

Dates et versions

hal-01366402 , version 1 (14-09-2016)

Identifiants

Citer

Emeric Miclet, Daniel Abergel, Aurélien Bornet, Jonas Milani, Sami Jannin, et al.. Toward Quantitative Measurements of Enzyme Kinetics by Dissolution Dynamic Nuclear Polarization. Journal of Physical Chemistry Letters, 2014, 5 (19), pp.3290-3295. ⟨10.1021/jz501411d⟩. ⟨hal-01366402⟩
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