Genomic and physiological analysis reveals versatile metabolic capacity of deep-sea Photobacterium phosphoreum ANT-2200. - CNRS - Centre national de la recherche scientifique Accéder directement au contenu
Article Dans Une Revue Extremophiles life under extreme conditions Année : 2016

Genomic and physiological analysis reveals versatile metabolic capacity of deep-sea Photobacterium phosphoreum ANT-2200.

Sheng-Da Zhang
  • Fonction : Auteur
Claire-Lise Santini
  • Fonction : Auteur
Wei-Jia Zhang
Valérie Barbe
Sophie Mangenot
  • Fonction : Auteur
Charlotte Guyomar
Marc Garel
Hai-Tao Chen
  • Fonction : Auteur
Xue-Gong Li
  • Fonction : Auteur
Qun-Jian Yin
  • Fonction : Auteur
Yuan Zhao
  • Fonction : Auteur
J. Armengaud
Jean-Charles Gaillard
  • Fonction : Auteur
Severine Martini
Nathalie Pradel
Claude Vidaud
  • Fonction : Auteur
François Alberto
Claudine Médigue
Long-Fei Wu

Résumé

Bacteria of the genus Photobacterium thrive worldwide in oceans and show substantial eco-physiological diversity including free-living, symbiotic and piezophilic life styles. Genomic characteristics underlying this variability across species are poorly understood. Here we carried out genomic and physiological analysis of Photobacterium phosphoreum strain ANT-2200, the first deep-sea luminous bacterium of which the genome has been sequenced. Using optical mapping we updated the genomic data and reassembled it into two chromosomes and a large plasmid. Genomic analysis revealed a versatile energy metabolic potential and physiological analysis confirmed its growth capacity by deriving energy from fermentation of glucose or maltose, by respiration with formate as electron donor and trimethlyamine N-oxide (TMAO), nitrate or fumarate as electron acceptors, or by chemo-organo-heterotrophic growth in rich media. Despite that it was isolated at a site with saturated dissolved oxygen, the ANT-2200 strain possesses four gene clusters coding for typical anaerobic enzymes, the TMAO reductases. Elevated hydrostatic pressure enhances the TMAO reductase activity, mainly due to the increase of isoenzyme TorA1. The high copy number of the TMAO reductase isoenzymes and pressure-enhanced activity might imply a strategy developed by bacteria to adapt to deep-sea habitats where the instant TMAO availability may increase with depth.
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Dates et versions

hal-01410136 , version 1 (06-12-2016)

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Sheng-Da Zhang, Claire-Lise Santini, Wei-Jia Zhang, Valérie Barbe, Sophie Mangenot, et al.. Genomic and physiological analysis reveals versatile metabolic capacity of deep-sea Photobacterium phosphoreum ANT-2200.. Extremophiles life under extreme conditions, 2016, 20 (3), pp.301-10. ⟨10.1007/s00792-016-0822-1⟩. ⟨hal-01410136⟩
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