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Article Dans Une Revue Journal of Cell Science Année : 2014

Synemin acts as a regulator of signalling molecules during skeletal muscle hypertrophy

Zhenlin Li
Ara Parlakian
Dario Coletti
  • Fonction : Auteur
Sonia Alonso-Martin
  • Fonction : Auteur
Christophe Hourde
  • Fonction : Auteur
Pierre Joanne
Jacqueline Gao-Li
  • Fonction : Auteur
Jocelyne Blanc
  • Fonction : Auteur
Arnaud Ferry
Denise Paulin
  • Fonction : Auteur
Zhigang Xue
  • Fonction : Auteur
Onnik Agbulut

Résumé

Synemin, a type IV intermediate filament (IF) protein, forms a bridge between IFs and cellular membranes. As an A-kinase-anchoring protein, it also provides temporal and spatial targeting of protein kinase A (PKA). However, little is known about its functional roles in either process. To better understand its functions in muscle tissue, we generated synemin-deficient (Synm(-/-)) mice. Synm(-/-) mice displayed normal development and fertility but showed a mild degeneration and regeneration phenotype in myofibres and defects in sarcolemma membranes. Following mechanical overload, Synm(-/-) mice muscles showed a higher hypertrophic capacity with increased maximal force and fatigue resistance compared with control mice. At the molecular level, increased remodelling capacity was accompanied by decreased myostatin (also known as GDF8) and atrogin (also known as FBXO32) expression, and increased follistatin expression. Furthermore, the activity of muscle-mass control molecules (the PKA RII alpha subunit, p70S6K and CREB1) was increased in mutant mice. Finally, analysis of muscle satellite cell behaviour suggested that the absence of synemin could affect the balance between self-renewal and differentiation of these cells. Taken together, our results show that synemin is necessary to maintain membrane integrity and regulates signalling molecules during muscle hypertrophy.

Dates et versions

hal-01545450 , version 1 (22-06-2017)

Identifiants

Citer

Zhenlin Li, Ara Parlakian, Dario Coletti, Sonia Alonso-Martin, Christophe Hourde, et al.. Synemin acts as a regulator of signalling molecules during skeletal muscle hypertrophy. Journal of Cell Science, 2014, 127 (21), pp.4589-4601. ⟨10.1242/jcs.143164⟩. ⟨hal-01545450⟩
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