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Article Dans Une Revue Journal of Cell Science Année : 1999

Rvs167p, the budding yeast homolog of amphiphysin, colocalizes with actin patches

Résumé

In this report, we have shown that the yeast amphiphysin-like protein Rvs167p was localized mainly in small cortical patches throughout the cell in unbudding cells. During budding, the patches were polarized at bud emergence site. During mating, Rvs167p was concentrated at the tip of the shmoo. Rvs167p colocalized with actin patches during yeast vegetative growth and mating. Complete disruption of the actin cytoskeleton using Latrunculin-A did not affect Rvs167p localization in patches throughout the cell. In rvs167 mutant cells, actin patches are mislocalized and in rvs167 or abp1 mutant cells, Rvs167p localization is not affected. These observations suggest that Rvs167p may localize the actin cortical complex properly. Finally, the amphiphysin-conserved N-terminal domain of Rvs167p, called the BAR domain, was required but not sufficient for the correct localization of the protein.
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Dates et versions

hal-01565053 , version 1 (19-07-2017)

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  • HAL Id : hal-01565053 , version 1

Citer

A. Balguerie, Pierre Sivadon, M. Bonneu, M. Aigle. Rvs167p, the budding yeast homolog of amphiphysin, colocalizes with actin patches. Journal of Cell Science, 1999, 112 (15), pp.2529-2537. ⟨hal-01565053⟩
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