Image-based fluorescence recovery after photobleaching (FRAP) to dissect vancomycin diffusion-reaction processes in Staphylococcus aureus biofilms - CNRS - Centre national de la recherche scientifique Accéder directement au contenu
Communication Dans Un Congrès Année : 2011

Image-based fluorescence recovery after photobleaching (FRAP) to dissect vancomycin diffusion-reaction processes in Staphylococcus aureus biofilms

Résumé

The diffusion capabilities of free fluorophores inside the heterogeneous three dimensional structure of Staphylococcus aureus biofilm were studied by an original image-based Fluorescence Recovery After Photobleaching method. The study was extended to BODIPY-vancomycin in order to better understand the mechanisms involved in the high tolerance of the bacteria embedded in a biofilm to the antibiotic.
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Dates et versions

hal-01601791 , version 1 (02-10-2017)

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S. Daddi Oubekka, Romain Briandet, F. Waharte, M.-P. Fontaine-Aupart, K. Steenkeste. Image-based fluorescence recovery after photobleaching (FRAP) to dissect vancomycin diffusion-reaction processes in Staphylococcus aureus biofilms. Conference on Clinical and Biomedical Spectroscopy and Imaging II, May 2011, Munich, Germany. ⟨10.1117/12.889461⟩. ⟨hal-01601791⟩
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