Detection of the multidrug resistance of P-glycoprotein in healthy tissues: the example of the blood-brain barrier - CNRS - Centre national de la recherche scientifique Accéder directement au contenu
Article Dans Une Revue Annales de Biologie Clinique Année : 1996

Detection of the multidrug resistance of P-glycoprotein in healthy tissues: the example of the blood-brain barrier

Résumé

The blood-brain barrier is formed by the cerebral capillary endothelial cells, joined together by tight junctions. These cells express the general endothelial cell markers as well as specific markers found on endothelial cells forming physiological barriers such as gamma-glutamyltranspeptidase, the glucose transporter Glut1 and the neutral amino-acid transporter. Using the monoclonal antibodies C219 and MRK16, we have revealed by Western blot and immuno-histochemistry the expression of the multidrug resistance P-glycoprotein on isolated rat cerebral cortex capillaries. On the other hand, P-glycoprotein was not detectable in brain cortex homogenates. P-glycoprotein thus appears to be a blood-brain barrier endothelium-specific marker which could regulate brain penetration of xenobiotics and thus participate in the neuroprotection of the brain.
Fichier non déposé

Dates et versions

hal-01606080 , version 1 (02-10-2017)

Identifiants

  • HAL Id : hal-01606080 , version 1
  • PRODINRA : 406337
  • PUBMED : 8731793
  • WOS : A1996UC21600008

Citer

Delphine Lechardeur, V. Phung-Ba, P. Wils, D. Scherman. Detection of the multidrug resistance of P-glycoprotein in healthy tissues: the example of the blood-brain barrier. Annales de Biologie Clinique, 1996, 54 (1), pp.31-36. ⟨hal-01606080⟩

Collections

CNRS
25 Consultations
0 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More